Modified Leeming and Notman Agar
Amy Gladfelter
Published: 2023-06-02 DOI: 10.17504/protocols.io.kxygxm3ywl8j/v1
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Abstract
Media recipe for growth of Malassezia species.
Before start
Attachments
Malassezia media_1.pdf
Steps
1.
bacteriological peptone (Oxoid) 10g
2.
yeast extract 2g
3.
desiccated ox bile (Oxoid) 8g
4.
glucose 10g
5.
glycerol monostearate 0.5g
6.
agar 15g
7.
glycerol, 50% 20mL
8.
tween 60 5mL
9.
olive oil 20mL
10.
dH2O 955mL
11.
Sterilize by autoclaving.
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