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    Open Field

    Haley Geertsma

    Published: 2022-03-01 DOI: 10.17504/protocols.io.b5qzq5x6
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    Abstract

    This protocol is used to test mice in the Open Field behavioural test.

    Steps

    1.

    Habituate mice in a separate room for 60 minutes prior to testing.

    2.

    Confirm testing room lighting is set to 100 lux and EthoVision is set up properly.

    3.

    Place mice into open field box and record their movement for 10 minutes.

    4.

    When testing is complete, place mice back into their home cage and clean the open field boxes well before testing the next mice.

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